Saturday, August 30, 2008
IM SO BORED!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
but i cant relli complain cuz this always happens -_-
sch = complain wish hols come...
hols come liao.... = complain bored.
hypocrisy sia...>.>
Tag rplies:
Yrgav: orh ok lor. lai ba!!!
Annabelle: off9 msg la. LOL
4:11 PM
Thursday, August 28, 2008
YO PPL! LOL been 2 busy/tired/sick lately 2 make a proper post. haha. these few days hav been @_@ cuz GRP1 has been preparing 4 their grp games!! =D
was supposed 2 go meet fellow GLs 4 meet dave movie outing but im SICK!! RAWRR!! sianded sia. so 2day i woke up at like 10? den eat breakfast den slp til 3.30. den eat lunch den i slp ntil now. im such a PIG =/
anw ytd games was successful! i think...=x hahaha i realize tat e SGLs r like um super tolerant? LOL cuz they organize e games 4 us evry week. n now we organize 1 time only n we r like @_@ alrdy. n its not easy....so muz say SGLs 谢谢！！！haha but mayb 4 us is tougher cuz our manpower is ridiculously LOW....>.> n we e 1st grp somemore seh! so GRP1 ppl oso muz say....我们是 ZAI KIA!!!!! =D
oh this happened ytd. it sorta made me realize tat im not as hot tempered as b4. =p
i was on e way home from GL training(taking bus). was super tired so i was slping. den this old eurasian lady with her luggage n all was walking 2 e door. den her luggage hooked on2 my leg. den she went like "fuck" n tried forcefully pulling her luggage. i think if i nvr lian wushu n was SLIGHTLY flexible, she sure pull ntil my leg sprain or break or sth. was damn pain la. den i juz unhook my leg from her luggage. den i heard her muttering. den she turn around n said 2 me " u fucking piece of shit" den i juz ignore her. but i was like thinking, mygod i dwan a eurasian wife sia. den i think she not happy i ignore her or sth, cuz she started yelling "wtf is wrong with u?!" while slapping my shoulder. den i felt damn pissed off n had a urge 2 punch her bony face. but she old lady...so i say nvm n juz glared at her. den 1 guy went n ask her wads wrong den she started bitching 2 him n ignore me. LUCKILY e guy turn her attn away sia. or i duno whether i can contd holding my temper. so dao mei lor meet this kind of woman. but this has shown tat my temper is improving! which is GOOD! haha.
ohya. on mon after going buy log stufs 4 grp1 games, went 2 meet ah jul rachacha n e XO yrgav =x haha was late by 1hr. LOL but nvm. cuz buying e log stuffs were kinda fun. haha ok lazy blog le...wana go slp agn. =p LOL
MY EYES ARE NOT SMALL!!!!
LOL so hard squeeze 4 ppl inside e screen. haha
tag rplies =)
Shuenyee : haha enjoying =)
Annabelle : haha shh secret. lol rply u on msn liao =)
zphy: LOL tats y im THINKIN of going...=/ hahaha lol dwan rabbit la. i think trilon wil eat it or sth =/ LOL
asyraff: haha thx raff!! LOL qte tough seh. haha
10:54 AM
Tuesday, August 26, 2008
i juz realized tat theres a sucky coach n im determined 2 overthrow him.
R.I.P ARSEHOLE
12:31 AM
Saturday, August 23, 2008
EXAMS ARE OVER!!!!!
WOOHOO! LOL ok i juz woke up after hibernation. LOL HMMMMMMMMMMMMM...............where 2 start....LOL YEA this morning last paper. start there ba. HAHAHHA i got like alot of random thoughts and i duno how 2 spill it all out O_O
hahaha this morning. i woke up got a SUPER BAD HEADACHE sia. i so enthu 4 microbio lor. wake up like 1hr early juz 2 go sch revise. LOL met bingxin n qiudong at e bus stop. bingxin is a crap qn asker lor! LOL below a few examples of his qns.
WHICH SCIENTIST WON A NOBEL PRIZE IN 1993?
THERE WAS AN OILSPILL AT ???? YEAR. WHAT IS THE NAME OF IT?
LOL total no link rite? bcome history exam. hahahhaha. den hor. duno y. i enter exam hall. bcome like SUPER HIGH. LOL den i realize i suddenly like super happy no worry lidat. LOL. den e few mins b4 e exams i crapped with yrgay and yingmin. LOL. den exam start hor. wah not bad sia. i feel PROUD!!!! of myself. HAHAHHA.
BUT
dr lisa HOHOHOHOHO absolutely REFUSES to let me b happy about at least 1 paper i did. she HAD 2 come out a dumb qns tats
1) not in notes
2)not in textbook
3)not in practical
4) was mentioned only once by her in lecture
5)all lectures tat Tan Hong Ming attends are his 1 way tickets 2 dream land
SOOOOOOOOOOO Tan Hong Ming didnt know how 2 do tat qns. N b4 tat he was SOOOOO happy with himself cuz he knew how 2 do all e qns n it was chicky peasy. den dr lisa HOHOHO went WAHAHAHAHA(evil laugh) whipped out her evil qns and stabbed Tan Hong Ming, our brave and noble prince, right in his PURE and GRACIOUS heart. LOL =x
THE QNS WORTH 10MARKS LOR!!!!!!!!!!!!!!!!!! sianded...>.>
LOL den this 2piddddddddddddd examiner. i finish e paper. so i put evryth nicenice. IM SUCH A NICE N TIDY PERSON!! bent down tie shoe lace. examiner come. she ask me.
"u finish?"
raises head and says "ya." bends back down.
"ok"
i tie finish. raise head agn. ans booklet gone. LOL i say finish paper doesnt mean finish checking RITE!!!! den nvmmmmm. i walk out. LOL at 1st stil quietquiet.....den i saw dan. den i start crap with him. "DISCUSS PAPER" LOL we went downstairs den evry1 like discuss e paper super long? LOL i duno y oso. i expected WOOHOO over liao! lets go celeb blabalablbalablablablabalb.
LOL den tat 2piddddd yrgay. organize go play pool. but HOR!! nvr say CLEARLY when. LOL den tat ah jul got datedate. so in end nvr go. den my HIGHNESS was dying down. so my SUPER BAD HEADACHE come back agn T_T so i go home..............
slpslpslsplspslpslpslsplsplsplspslpslsplspslpslpslsplsplspslpslsplspslpslpslsplsplspslpslsplsplspslp
slpslsplsplspslpslsplspslpslpslsplsplspslpslpslpslsplspslpslpslspslplpslsplsplspspslpslpslsplsplspls
LOL from slp --> pls LOL
NVM
NOW IS HOLS!
WAD SHUD I DO??!!!
hmmm okok i shall plan NOW!
lets ccc........
1) train 4 IVP
2)MAYB organize a class outing.
3)MAYB fulfil my CA obligations. LOL i feel damn guilty bout this CA 1 lor =/ cuz i like signed up 4 e workshop during Os period? LOL e hols la. den i nvr go help out de...=p LOL sooo mayb i shall go back help out? hahaha night safari CA! LOL hmmm den while im there can go e ZOO! lol v long nvr go zoo liao sia. hahaha.
4)umm pack my room? LOL but actually this is a no use thing cuz 100% 1day after i pack its back 2 its original state. hahahahha
haha i think tats all. LOL all tat i can think of 4 now. wah seh i think hols gona b damn fun lor! LOL got OTC got go sp training. woohoo got alot things sia. ESP looking 4ward 2 OTC sia. hahaha its like gona b....
F?
U?
N?
FUN?
hahahhaha ok i wana slp agn. LOL byebye
Tag rplies =D
Yrgay:IS TYPO LOR!
Gordon: hahahha u 2! WOOHOO SIA!
SY: YES! =) LOL time 2 sit back relax n .........do w/e i 1!! =}
12:42 AM
Thursday, August 21, 2008
CLOSE!!! ABIT MORE ONLY!!! JIAYOU AHHHHHH!!!!!!
left with bacteria and cell wall only. so i shall rest ntil 3pm. den study bacteria. den after finish bacteria i rest another 1hr. den study cell wall. den after tat i revise evryth agn ntil around 11pm? den slp. den tmr....ENDURE 1 more paper. and den...................
FREEDOM 4 2MTHS!!!!!! WAHHHOOOOOOO
1:59 PM
Wednesday, August 20, 2008
brain saturation point reached....DAMN
10:02 PM
YO PPL! LOL gona b a boring post unless u super duper interested in microbiology!!! hahhahaha sooooooo here goes!!!~~~
OK 1ST IS!!!! sterilization and disinfection!!!!
S: sterilization
Eliminates ALL microorganisms and microbes on or in an object.
Autoclave/ethylene oxide gas
Used 4 sterilizing canned FOOD!!! ,Surgical instruments, culture media
D: disinfection
Eliminates most microorganisms
Alcohol
Disinfects workbenches 4 preparation of culture media
A: antisepsic
Eliminates most microoganisms on living tissue
Alcohol. Iodine(antiseptic)
Prevents infections of open wounds.
P: pasteurization
Eliminates microoganisms, preventing change of taste and nutrients
Heat 4 a short period
Pasteurization of milk and fruit juices etc
how does soap wash away microbes on or hands?
soap molecules hav a fatty end and a ionic end. when mixed with water it creates a suspension when e water binds 2 e ionic end and e oil bind 2 e fatty end. this allows water 2 wash away e microoganisms on our hands.
how does antimicrobials work.
3 types: bacteriostatic, bacteriocidal, bacteriolytic
bacteriocidal kills e living cells.bacteriolytic kills off e cells and destroys them.( seen by total cell count sloping down along with the living cell count in graph)
antifungal drugs.
polyene antibiotics prevent ergosterol in e cell membrane. ergosterol in fungi but cholesterol in humans.
imidazole and triazole inhibit ergosterol synthesizing enzymes, preventing formation of cell membrane.
NEXT OFF IS MICROBIAL ENVIRONMENTAL FACTORS!!!
5: temp, pH, water availability, oxygen, UV light.
with temp: psychrophiles( loves low temp), mesophiles( normal temp), thermolphiles( high temp like duh -_-)
when below minimum temperature, cell membrane has 2 little fluid and so no diffusion/transport of molecules; thus there is no growth.
when at optimum, the diffusion/transport of molecules is e highest and thus has highest growth.
when nearing maximum/above maximum, the high heat denature e cell proteins/membranes causing it 2 lyse/MATI!!!!
with pH: acidophiles, neutrophiles, alkalinophiles. if i need 2 explain these den uhhhh -_-
during bacterial growth:
protein breakdown increases pH.
organic acid production decreases pH
buffers help provide optimum pH 4 growth.
2 types of food poisoning: intoxications and infections
Intoxications: when food is left in e fridge 4 2 long, e bacteria leave toxins on e food, which causes u 2 go @@ if u nvr cook e food well enough b4 eating.
Infections: whereby e bacteria grow in ur digestive tract! ewwwwwwwww LOL
3 types of infections: bacterial, viral, parasitic
salmonella ella ella eh eh under my umbrella is e 2nd most common bacterial infection.
all salmonella inffections are called salmonellosis except 4 an infection by salmonella typh which is called typhoid fever.
salmonella mostly found in raw/undercooked eggs poultry meat fish shellfish such as shrimp BLABLABLABLABLABLABLABLABLABLABLABLABLABLABLA
e most common type of viral infection is hepatitis A. which is due 2 water getting contaminated by raw sewage, shellfish tat r harvested from contaminated water.
foods tat hav been known 2 contain hepatitis A: water, sliced luncheon meat, sandwiches, SALADS( I KNEW VEGES ARE BAD!!!!!!)
parasitic infections: mostly caused by trichinella spiralis which causes u 2 hav trichinosis.
infects pigs bears etc.
we get infected due 2 undercooked pork etc.
NOW E 3RD FACTORS ISSSSSSSSSSSSSSSSSSSSSSSSS WATER AVAILABILITY!!!!!
microorganisms grow in diff water availability.
Halophiles( love high salt conc. )
Osophiles( love high sugar conc.)
xerophiles(love dryness)
how do microbes adapt 2 low water availability?
method1: they pump in inorganic ions such as K+
method 2: they store water soluble molecules such as sucrose.
industrial applications of e above shit:
PCR(polymerase chain reaction)
uses heat stable enzymes found in thermophiles 2 rapidly amplify DNA. process is called taq polymerase. this won Kary mullis e nobel prize in 1993.
Bioremediation: this uses e enzymes from bacteria, algae, plants etc 2 change an evironment tat has been contaminated back 2 its original state. (eg. oil spills)
NEXT ISSSSSSSSSS MICROBIAL NUTRITION AND GROWTH!
life needs : energy, CHNOPS, water 2 survive.
2 types of energy sources: chemotroph and phototroph.
2 types of chemotrophs: chemoorganotrophs and chemolithotrophs.
chemotrophs use chemotrophy 2 get energy from organic/inorganic substances.
chemoorganotrophs use organic substances such as glucose/sucrose etc.
chemolithotrophs use inorganic substances such as H and C
phototrophs use phototrophy 2 get energy from sunlight.
Auto and heterotrophs: autotrophs use CO2 as their source of carbon while heterotrophs use other sources as their carbon source.
OXYGEN!!
aerobes and anaerobes
aerobes need oxygen 2 survive.
anaerobes need 2 hav no oxygen 2 survive
facultaive anaerobes can survive in both
micro aerophiles can survive in regions of low oxygen conc.
obligate and facultative
obligate: must have either oxygen/ NO oxygen at all 2 survive
facultative: flexible
why obligate anaerobes canot survive in oxygen?
oxygen changes in2 molecules in cells:
superoxide free radical: O2-
hydrogen peroxide: H2O2
hydroxyl radical: OH.
these molecules can take electrons from cell proteins etc. therefore cell will die.
aerobes can survive due 2 e presense of superoxide dimutase(SOD) and catalase.
superoxide dismutase converts superoxide free radicals in2 hydrogen peroxide and O2
catalase converts H2O2 in2 water and oxygen.
GROWTH GRAPH
consists of : rest, lag, exponential, stationary, death phases.
lag phase: synthesis enzymes, cell repair, change of cell chemistry, germination from rest phase.
exponential: cells divide rapidly.
Stationary: some cells start 2 die. (accumulation of wastes/lack of food/compt 4 food)
death: more cells mati-ing den XXX-ing and so less cells. mati-ing due 2 e loss of ability 2 detoxify toxins as wastes accumulate. food running out.
Culture media: 2 types
synthetic and nonsynthetic.
synthetic has known ingredients and amounts.
nonsynthetic has unknown amounts and ingredients
Calculation of growth: Nf= Ni(2^n)
where Nf is final cell number
Ni is initial cell count
n is number of generations
2 calculate n, times the time allowed 2 grow with time taken 2 undergo binary fission once.
measuring microbial growth:
cell numbers: viable and total
cell mass
viable: spread and pour plates
spread plates: culture is pipetted on2 media and spread evenly. incubate and den count number of colonies.
Advantages counts living cells only
disadvantages: strictly 4 aerobes. microbes diffuse and colonies may overlap.
Pour plates: culture is pipetted on2 petri dish. media is poured in and mixed well with culture. incubate and then count.
advantages: counts living cells only.
disadvantages: strictly 4 anaerobes only, colonies are smaller as movement is restricted and counting is difficult.
Cell mass: centrifuge and filtraion
centrifuge:
pour culture in2 centrifuge tube and centrifuge. pour off top layer. and add in distilled water. centrifuge agn. pour off top layer agn. dry e tube and weigh e cells left bhind.
filtration:
weigh e filter paper. pour in a known amount of culture. wash filtrate with distilled water. let it dry.
filtration is more accurate den centrifuge.
DWAN TYPE LIAO!!! HAND IS SUPER E TIRED!!!! ARGHHHH
7:22 PM
Friday, August 15, 2008
OMG!!! NP got kidnapper seh! so scary sia! n on e day tat NWC start somemore sia!! SCARYYYY. ANW 2day i got lotsa pics from e 1st day of NWC!! LOL
LOL u c me n liting. SO GUAI SIA!! bring notes 2 mug during e compt. HAIZ. we 2 guai liao la. c me mugging ipc. LOL
This yuling ah. i mugging den she come disturb me 1 me take this shuai ge pic. 2pid seal sia!
wah u c this elvis n kelvin. compt liao stil 1 fight with each other! dun fite la....save ur strength fite other ppl!!!! LOL
c ziwei act cute buay cute. LOL
YULING FORCE ME DE!! make me make retard face. haiyoyo
u c this mad woman. snatching 4 e camera. haiz luv it so much sia.
u c e ghost at e background. SCARY SEH. NPWushu team kena haunted le. =((
we finally decided uncle zhaowei 2 poor thing la. 2 uncle liao always dun take pic with him. soooo ok la let u take 1 with us. LOL
zhaowei decided 2 try 2 rape us.
NPWushu team swannnnn!! LOL pink color....eeeeeeeeeeee. haha
some1 candid me from behind. =.-
all the competitors. hahaha. so colorfullll. LOL
ok la. stil got somemore sia. LOL another day den post. my video oso another den post. tired liao. haha
Tag rplies =)
Checktime: @_@ lol who r u
Gabriel: LOL poly life now super fun sia! LOL u next yr come poly lor. waste 1 yr of ur life only. haha. dun emo hor. =)
11:17 PM
Wednesday, August 13, 2008
YO! TMR START NWC LE!! evry1 gona hav fun ok!! WAHAHAHA. i tmr hope all e judges r female cuz if all female sure all fall 4 my hotness n gime 10!!!! =p LOL im so thick skin i think HAHHAA
LOL ok such a short post. LOL
tag rplies =D
kt: U WERE DYING OSO LOR PLZ!!!
raff: thx raff =) haha u oso hor!
SASA: hahaha i wish u gd luck in not breaking ur calculator b4 exams sia! LOL
Wenliang: LOL means ur chi poor oso!! YAY =D
Fanny: no la! where got so pro sia...is HOPE only =x
12:09 AM
Tuesday, August 12, 2008
哈哈哈！！！ 大家好！！！大家都很surprised 吧！！ 因为我在用华文blog!!!
哈哈哈。。。比赛快要到了！！我还没准备好。。但是没关系。。因为肯定会很
好玩的！！@@用华文blog很难啊！海！！ 今天。。finally考完一个module
了！！ 好开心。因为我觉得很容易！！ 哈哈哈！！ 希望能得到AD!! （在发
呆）=p 海！！ 好了！！ 就这样吧！用华文blog真难啊！！
啊！是的！要给sasa =)一个大谢谢！哈哈教我how to blog in 华文！
哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈
12:31 AM
Sunday, August 10, 2008
Physio CT:
Blood: DONE!
Heart: DONE!
Respiratory Systems: DONE!
Digestive System: DONE!
Urinary System: DONE!
Sexual Reproduction AKA XXX: DOne(means half done LOL)
HAHA i juz cant resist posting this crap shi.LOL cuz i've been mugging since like i reached back home? which was like at 6+? n now is 12+!!! MY LONGEST STREAK EVR!!!!~!!! LOL
anw 2day went 2 watch The Mummy!! O_o LOL bought myself a hotdog n spammed e tomato n mayonnaise n made it look like shit agn! LOL i luv doing tat la. cuz it tastes freaking nice. LOL u cant taste e bread anymore. cuz its like soaked? HAHAH
okok back 2 mugging fever!! BUT 1st!!! IM HUNGRY!!! so fridge here i come!!!! RAWRRRR *drools*
12:06 AM
Friday, August 08, 2008
National Wushu & Sanshou Championship 2008 will be held in Toa Payoh Sports Hall on 11-17 August 2008. Tickets prices: $5.00 (Monday-Friday), $10.00 (Saturday-Sunday). Tickets are now for sale at Singapore National Wushu Federation. Please get your tickets early to avoid disappointment.
i have only 1 thing 2 say.
FLUORINE URANIUM CARBON POTASSIUM
go figure !@#$%^&
1:01 AM
Wednesday, August 06, 2008
HOLY SHIT
SINCE WHEN WAS COMPT NEXT WEEK
IM SO LIKE NOT READY??!!
SIAO LIAO LA MUZ CHIONG SIA
OMG LIDAT HOW STUDY PHYSIO!!! MY FAV MODULE SOMEMORE
ARGHHHHHH SHITSHITSHITYSHITILYSHIT
TOTALLY MUZ REMAKE MY STUDY PLAN!! (yes i hav 1 cuz i like np n so i wana do well lol =p)
!@#$%^&*()(*&^!@#$%^&&!&*()(*&()(%^*()_)(^%$#@#$%^&*()_(@#$%^&*()_%$@!
12:09 AM
Monday, August 04, 2008
YO!! LOL so gd sia 2day no sch leh. but went back sch 2 discuss LSCT grp games things!!
ONLY ME N CAILING SHOWED UP!!!! VANESSA N AMANDA DISAPPEARED!!! DD=
BUT we finalized e thing!!! WOOHOOO!! now waiting 4 amanda's idea only. haha. omg i think we damn pro la. 1time meeting can do so much thing. so zai. anw after e meeting i stay bhind n chatchat with ziwei n shawn. haha cuz i so bored come home oso nth 2 do. LOL. den after i came home. i was like "ok study physio!!!!!" i took out e notes. i read 4 10mins.........................................
i fell aslp.
LOL slp ntil like 9? lol i 4got. nvr look at clock.
haha den sth funny/zzzz happen during training on fri.
Coach: whats ur chi name?
Me: tels him how 2 write**
Coach: ur age?
Me: 16
Coach: 26??
Me: 16 not 26.
Coach: serious? i tot u 20+ leh
Me: -_-ll i 17 this yr not yet bdae so 16 only.
Coach: laughs*
Me: i where got look so old sia -_-lll
LOL like @@" rite? i where got look like 20++...omg make me sound like i look v old lidat... i so sad lor T__T ohwell...
11:02 PM
Friday, August 01, 2008
This post has been edited under request of *AHEM* LOLL
YO ppl! LOL shall post bout e last GL training b4 e hols! HAHHAA super fun this week. N I HAV PROOF!! LOL this happened in e bathrooms(MALE) after e training. when we were all bathing! HAHAH enjoy!!! LOL i draw myself 1 ok!
LOL can any1 guess who is this?? HAHAHA
Jamie n Renee r so scary T__T guys = peeping tom...so girls = peeping ???
LOL he walk til v relax lor! hahahha
LOL now not relax liao!! HAHAHA
had 2 make sure all e guys were wearing clothes 1st. LOL cant let them peep at our sexy bodies!!! HAHHA
Bingxin finally got his bath!! haha he waited vvv long lor! hahaha kp shout i 1 2 BATH!!! LOL
This EXTRAORDINARY event occurred approximately 5mins after Bingxin bathed finished. u can estimate e amount of time he spent inside ba. HAHHAHA
anw tat training rocked sia =D haha BUT HOR!!!! e flour thingy game hor!!! LOL they waste all e flour on us!! den e last 2 grp nvr tio alot!! RAWRRR not fair!! LOLL! duno how ration e flour...tsktsk. den after e games we got debrief by shane. haha i think he rocks. LOL hmm...lets juz say he know juz e rite amt of force 2 use. =)
This was from a few trainings ago. plz take a gd note of bingxin's expression! LOL n charmaine looks so aunty =p
2pid yongjinz....try scandal me!! RAWRRRR
11:50 AM
